Associate Professor Wayne Greene
B.Sc (Hons), Ph.D

Associate Professor in Molecular Genetics

About me

I am an Associate Professor in Molecular Genetics and have been a staff member within the School of Veterinary and Biomedical Sciences since 1998. I graduated with a B.Sc with Honours in Pathology from the University of WA in 1987, and then completed a Ph.D in Microbiology at UWA in 1990. Initially, I undertook postdoctoral positions within the Veterinary School at Murdoch University. I then moved to Singapore where I gained experience working in the medical biotechnology sector. After being awarded an NHMRC CJ Martin fellowship, I travelled to the UK for a postdoctoral position at the Medical Research Council Laboratory of Molecular Biology in Cambridge. Upon my return to Perth, I worked as a postdoctoral scientist at the Telethon Institute for Child Health Research prior to my current appointment. I am now Academic Chair of the Biomedical Science program, coordinator of the capstone Biomedical unit “Advances in Medical Science” and I teach molecular genetics to Biomedical Science and Veterinary undergraduates. My research interests are varied, but share a common molecular theme.

Teaching area

I currently teach:

1. Molecular genetics to Biomedical Science and Veterinary Science students through the unit “Immunology and Molecular Genetics”.

2. Molecular techniques and cancer biology to Biomedical Science students through the unit “Advances in Medical Science”.

3. Role of genes in development to Veterinary Science students through the unit “Veterinary Physiology II”.

Research areas

1. Molecular basis of human leukaemia.

2. Molecular genetic profiling of canine lymphoma.

3. Molecular mechanisms of evolution: Role of transposable DNA elements.

4. Molecular approach to flea control by vaccination.

Awards and grants

I received a Vice Chancellor’s Award for Teaching Excellence in 2011.

Professional and community service

1. Major administrative duties include 
Academic Chair of Biomedical Science, Member of the School Board, Member of the Veterinary Science Curriculum Committee, Member of the Animal Science Curriculum Committee, Member of the Honours Subcommittee.

2. Peer reviewer of nationally competitive grants including NHMRC Project Grants.

3. Peer reviewer for international scientific journals including Nature Medicine, Genes, Chromosomes and Cancer, British Journal of Haematology, FEBS Letters.

4. Ph.D thesis examiner.

5. Provision of professional training to secondary school teachers and career advisors.

Doctoral and masters supervisions

Completed:

Heidari, Mansour (2003) Investigation of the molecular function of the nuclear oncoprotein HOX11 in human T-cell leukaemia. PhD thesis, Murdoch University.

Rice, Kim Lee (2004) Functional analysis of the HOX11 target genes ALDH1A1 and FHL1. PhD thesis, Murdoch University.

Dixon, Darcelle Natalie (2004) Identification of downstream target genes of the T cell oncoprotein HOX11 by global gene expression profiling. PhD thesis, Murdoch University.

Longville, Brooke Allison Cattell (2009) Genetic epidemiology of age-related macular degeneration (AMD): The role of the complement component 2 (C2) and complement factor B (CFB) genes in determining AMD subphenotypes. PhD thesis, Murdoch University.

Welch, Mathew David (2011) Molecular mechanisms underlying aberrant expression of the connective tissue growth factor in paediatric pre-B cell acute lymphoblastic leukaemia. Ph.D thesis, Curtin University.

 

Current:

Sharp, Margaret. Differential gene expression studies of canine lymphoma. PhD candidate, Murdoch University.

Oliver, Keith. The dynamic eukaryote genome: Evolution, and the TE-Thrust hypothesis. PhD candidate, Murdoch University.

Ng, Yoke Leng. The role of SPARC in intestinal extracellular matrix changes and inflammation. PhD candidate, Murdoch University.

Stayt, Jason. Identification and characterisation of specific genetic markers in the diagnosis of canine lymphoma. Masters candidate, Murdoch University.

Oksa, Annika. Classification and prognosis of canine lymphoma. Masters candidate, Murdoch University.

Publications

Hu, D., Nicholls, P.K., Yin, C., Kelman, K.R., Yuan, Q., Greene, W.K., Shi, Z. and Ma, B. (2018) Immunofluorescent Localization of Non-myelinating Schwann Cells and Their Interactions With Immune Cells in Mouse Thymus.Journal of Histochemistry & Cytochemistry, 66 (11). pp. 775-785.

Ma, B., Yin, C., Hu, D., Newman, M.Nicholls, P.K., Wu, Z., Greene, W.K. and Shi, Z. (2018) Distribution of non-myelinating Schwann cells and their associations with leukocytes in mouse spleen revealed by immunofluorescence staining. European Journal of Histochemistry, 62 (2). pp. 33-42.

Anderton, R.S., Hill, L.R., Morris, R., Mastaglia, F.L.Greene, W. and Boulos, S. (2017) Lack of evidence for decreased protein stability in the 2397 (Met) haplotype of the leucine rich repeat kinase 2 protein implicated in Parkinson’s disease. Advances in Parkinson’s Disease, 06 (04). pp. 113-123.

Shi, Z., Greene, W.K.Nicholls, P.K., Hu, D., Tirnitz-Parker, J.E.E., Yuan, Q., Yin, C. and Ma, B. (2017) Immunofluorescent characterization of non-myelinating Schwann cells and their interactions with immune cells in mouse mesenteric lymph node. European Journal of Histochemistry, 61 (3). pp. 193-203.

Ng, I., Fairchild, T.J.Greene, W.K. and Hoyne, G.F. (2016) Preferential mobilization and egress of Type 1 and Type 3 innate lymphocytes in response to exercise and hypoxia. Immunome Research, 12 (2).

Greene, W.K., Macnish, M.G., Rice, K.L. and Thompson, R.C.A. (2015) Identification of genes associated with blood feeding in the cat flea, Ctenocephalides felis. Parasites & Vectors, 8 (1). Article 368.

Welch, M.D., Howlett, M., Halse, H.M., Greene, W.K. and Kees, U.R. (2015) Novel CT domain-encoding splice forms of CTGF/CCN2 are expressed in B-lineage acute lymphoblastic leukaemia. Leukemia Research, 39 (8). pp. 913-920.

Longville, BAC, Anderson D, Welch MD, Kees UR and Greene, WK. (2015) Aberrant Expression of Aldehyde Dehydrogenase 1A Subfamily Genes in Acute Lymphoblastic Leukaemia is a Common Feature of T-lineage Tumours. British Journal of Haematology. 168 pp. 246-257.

Oliver, K., McComb, J. and Greene, WK. (2014) Jumping genes and the spectacular evolution of flowering plants. Australasian Science, March pp. 19-21.

Oliver, K.R.McComb, J.A. and Greene, W.K. (2013) Transposable elements: Powerful contributors to angiosperm evolution and diversity. Genome Biology and Evolution, 5 (10). pp. 1886-1901.

Ng YL, Klopcic B, Lloyd F, Forrest C, Greene WK, Lawrance IC. (2013). Secreted Protein Acidic and Rich in Cysteine (SPARC) Exacerbates Colonic Inflammatory Symptoms in Dextran Sodium Sulphate-Induced Murine Colitis. PLoS One 8(10). e77575.

Welch MD, Greene WK and Kees UR. (2013). Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia. British Journal of Haematology 162(4). pp. 537-541.

Oliver K.R. and Greene W.K. (2012). Transposable elements and viruses as factors in adaptation and evolution: an expansion and strengthening of the TE-Thrust hypothesis. Ecology & Evolution, 2 (11). pp. 2912-2933.

Oliver, K. and Greene, W. (2012) Jumping genes: How they drove primate evolution. Australasian Science, Jan/Feb . pp. 18-21.

Nilforoushan, N., Aghapour, S., Raoofian, R., Rad, S.S., Greene, W.K., Fakhraie, G. and Heidari, M. (2012) Lack of association between the C677T single nucleotide polymorphism of the MTHFR gene and glaucoma in Iranian patients. Acta Medica Iranica, 50 (3). pp. 208-212.

Youssefian, L., Vahidnezhad, H., Raoofian, R., Greene, W.K. and Heidari, M. (2012) Potential functions of the human homeobox TGIFLX/Y genes in normal and abnormal development. European Journal of Clinical & Medical Oncology, 4 (1).

Oliver, K.R. and Greene, W.K. (2011) Mobile DNA and the TE-Thrust Hypothesis: Supporting Evidence from the Primates. Mobile DNA, 2 (1). p. 8.

Saee-Rad, S., Hashemi, H., Miraftab, M., Noori-Daloii, M.R., Chaleshtori, M.H., Raoofian, R., Jafari, F., Greene, W.K., Fakhraie, G.,Rezvan, F. and Heidari, M. (2011) Mutation analysis of VSX1 and SOD1 in Iranian patients with keratoconus. Molecular Vision , 17 . pp. 3128-3136.

Anderton, R.S., Meloni, B.P., Mastaglia, F.L., Greene, W.K. and Boulos, S. (2011) Survival of motor neuron protein over-expression prevents calpain-mediated cleavage and activation of procaspase-3 in differentiated human SH-SY5Y cells. Neuroscience, 181 . pp. 226-233.

Milech, N., Gottardo, N.G., Ford, J., D’Souza, D., Greene, W.K., Kees, U.R. and Watt, P.M. (2010) MEIS proteins as partners of the TLX1/HOX11 oncoprotein. Leukemia Research, 34 (3). pp. 358-363.

Ousati Ashtiani, Z., Ayati, M., Modarresi, M.H., Raoofian, R., Sabah Goulian, B., Greene, W.K. and Heidari, M. (2009) Association of TGIFLX/Y mRNA expression with prostate cancer. Medical Oncology, 26 (1). pp. 73-77.

Oliver, K.R. and Greene, W.K. (2009) The Genomic Drive hypothesis and punctuated evolutionary taxonations, or radiations. Journal of the Royal Society of Western Australia, 92 (4). pp. 447-451.

Oliver, K.R. and Greene, W.K. (2009) Jumping genes drive evolution. Australasian Science, Sept . pp. 29-31.

Rice, K.L.Heidari, M.Taplin, R.Kees, U.R. and Greene, W.K. (2009) Transcriptional regulation of the human ALDH1A1 promoter by the oncogenic homeoprotein TLX1/HOX11. Hematology Reports, 1 (2). pp. 70-79.

Oliver, K.R. and Greene, W.K. (2009) Transposable elements: powerful facilitators of evolution. BioEssays, 31 (7). pp. 703-714.

Rice, K.L.Izon, D.J., Ford, J., Boodhoo, A., Kees, U.R. and Greene, W.K. (2008) Overexpression of stem cell associated ALDH1A1, a target of the leukemogenic transcription factor TLX1/HOX11, inhibits lymphopoiesis and promotes myelopoiesis in murine hematopoietic progenitors. Leukemia Research, 32 (6). pp. 873-883.

Rice, K.L., Kees, U.R. and Greene, W.K. (2008) Transcriptional regulation of FHL1 by TLX1/HOX11 is dosage, cell-type and promoter context-dependent. Biochemical and Biophysical Research Communications, 367 (3). pp. 707-713.

Dixon, D.N., Izon, D.J., Dagger, S., Callow, M.J., Taplin, R.H., Kees, U.R. and Greene, W.K. (2007) TLX1/HOX11 transcription factor inhibits differentiation and promotes a non-haemopoietic phenotype in murine bone marrow cells. British Journal of Haematology, 138 (1). pp. 54-67.

Greene, W.K., Sontani, Y., Sharp, M.A.Dunn, D.S., Kees, U.R. and Bellgard, M.I. (2007) A promoter with bidirectional activity is located between TLX1/HOX11 and a divergently transcribed novel human gene. Gene, 391 (1-2). pp. 223-232.

Heidari, M.Rice, K.L.Phillips, J.K.Kees, U.R. and Greene, W.K. (2006) The nuclear oncoprotein TLX1/HOX11 associates with pericentromeric satellite 2 DNA in leukemic T-cells. Leukemia, 20 (2). pp. 304-312.

Hoffmann, K., Dixon, D.N., Greene, W.K., Ford, J., Taplin, R. and Kees, U.R. (2005) Erratum: A microarray model system identifies potential new target genes of the proto-oncogene HOX11 (Genes, Chromosomes and Cancer (2004) 41 (309-320)). Genes, Chromosomes and Cancer, 42 (2). p. 210.

Hoffmann, K., Dixon, D.N.Greene, W.K., Ford, J., Taplin, R. and Kees, U.R. (2004) A microarray model system identifies potential new target genes of the proto-oncogene HOX11. Genes, Chromosomes and Cancer, 41 (4). pp. 309-320.

Watt, P.M., Hoffmann, K., Greene, W.K., Brake, R.L., Ford, J. and Kees, U.R. (2003) Specific alternative HOX11 transcripts are expressed in paediatric neural tumours and T-cell acute lymphoblastic leukaemia. Gene, 323 (1-2). pp. 89-99.

Greene, W.K., Ford, J., Dixon, D.Tilbrook, Peta A., Watt, P.M., Klinken, S.P. and Kees, U.R. (2002) Enforced expression of HOX11 is associated with an immature phenotype in J2E erythroid cells. British Journal of Haematology, 118 (3). pp. 909-917.

Heidari, M.Rice, K.L., Kees, U.R. and Greene, W.K. (2002) Expression and purification of the human homeodomain oncoprotein HOX11.Protein Expression and Purification, 25 (2). pp. 313-318.

Greene, W.K., Baker, E., Rabbitts, T.H. and Kees, U.R. (1999) Genomic structure, tissue expression and chromosomal location of the LIM-only gene, SLIM1. Gene, 232 (2). pp. 203-207.

Masson, N., Greene, W.K. and Rabbitts, T.H. (1998) Optimal activation of an endogenous gene by HOX11 requires the NH 2- terminal 50 amine acids. Molecular and Cellular Biology, 18 (6). pp. 3502-3508.

Greene, W.K., Bahn, S., Masson, N. and Rabbitts, T.H. (1998) The T-cell oncogenic protein HOX11 activates Aldh1 expression in NIH 3T3 cells but represses its expression in mouse spleen development. Molecular and Cellular Biology, 18 (12). pp. 7030-7037.

Deplazes, P.Penhale, W.J.Greene, W.K. and Thompson, R.C.A. (1995) Effect on humoral tolerance (IgG and IgE) in dogs by oral administration of ovalbumin and Der p I. Veterinary Immunology and Immunopathology, 45 (3-4). pp. 361-367.

Greene, W.K., Cheong, M.K., Ng, V. and Yap, K.W. (1995) Prevalence of hepatitis C virus sequence variants in south-east Asia. Journal of General Virology, 76 (1). pp. 211-215.

Kyaw-Tanner, M.T., Greene, W.K., Park, H. and Robinson, W.F. (1994) The induction of in vivo superinfection and recombination using feline immunodeficiency virus as the model. Archives of Virology, 138 (3-4). pp. 261-271.

Greene, W.K., Carnegie, R.L., Shaw, S.E., Thompson, R.C.A. and Penhale, W.J. (1993) Characterization of allergens of the cat flea, Ctenocephalides felis: detection and frequency of IgE antibodies in canine sera. Parasite Immunology, 15 (2). pp. 69-74.

Greene, W.K.Meers, J., del Fierro, G., Carnegie, P.R. and Robinson, W.F. (1993) Extensive sequence variation of feline immunodeficiency virusenv genes in isolates from naturally infected cats. Archives of Virology, 133 (1-2). pp. 51-62.

Meers, J., del Fierro, G.M., Cope, R.B., Park, H.S., Greene, W.K. and Robinson, W.F. (1993) Feline immunodeficiency virus infection: plasma, but not peripheral blood mononuclear cell virus titer is influenced by zidovudine and cyclosporine. Archives of Virology, 132 (1-2). pp. 67-81.

Greene, W.K.Penhale, W.J. and Thompson, R.C.A. (1993) Isolation and in vitro translation of messenger RNA encoding allergens of the cat flea, Ctenocephalides felis. Veterinary Immunology and Immunopathology, 37 (1). pp. 15-23.

Greene, W.K.Meers, J., Chadwick, B., Carnegie, P.R. and Robinson, W.F. (1993) Nucleotide sequences of Australian isolates of the feline immunodeficiency virus: comparison with other feline lentiviruses. Archives of Virology, 132 (3-4). pp. 369-379.

Morgan, U.M.Constantine, C.C.Greene, W.K. and Thompson, R.C.A. (1993) RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data. Transactions of the Royal Society of Tropical Medicine and Hygiene, 87 (6). pp. 702-705.

Lawson, M.Meers, J.Blechynden, L.Robinson, W.Greene, W. and Carnegie, P. (1993) The detection and quantification of feline immunodeficiency provirus in peripheral blood mononuclear cells using the polymerase chain reaction. Veterinary Microbiology, 38 (1-2). pp. 11-21.

Greene, W.K. and Thomas, W.R. (1992) IgE binding structures of the major house dust mite allergen DER P I. Molecular Immunology, 29 (2). pp. 257-262.

Greene, W.K., Cyster, J.G., Chua, KY, O’Brien, R.M. and Thomas, W.R. (1991) IgE and IgG binding of peptides expressed from fragments of cDNA encoding the major house dust mite allergen Der p I. The Journal of Immunology, 147 (11). pp. 3768-3773.

Chua, K-Y, Greene, W.K., Kehal, P. and Thomas, W.R. (1991) IgE binding studies with large peptides expressed from Der p II cDNA constructs. Clinical & Experimental Allergy, 21 (2). pp. 161-166.

Thomas, W., Chua, K-Y and Greene, W.K. (1990) Allergy to the house dust mite. Today’s Life Science, 2 (8). pp. 20-28.

Greene, W.K., Chua, KY, Stewart, G.A. and Thomas, W.R. (1990) Antigenic analysis of group I house dust mite allergens using random fragments of Der p I expressed by recombinant DNA libraries. International Archives of Allergy and Immunology, 92 (1). pp. 30-38.